Gintonin, an exogenous ginseng-derived LPA receptor ligand, promotes corneal wound healing

Title
Gintonin, an exogenous ginseng-derived LPA receptor ligand, promotes corneal wound healing
Authors
임혜원김현중김준영이병환최선혜김형춘안성엽정순욱장민희조익현나승열
Issue Date
2017-09
Publisher
JOURNAL OF VETERINARY SCIENCE
Citation
VOL 18, NO 3-397
Abstract
Ginseng gintonin is an exogenous ligand of lysophosphatidic acid (LPA) receptor. Accumulating evidence shows LPA helps a rapid recovery of corneal damage. The aim of this study was to evaluate the therapeutic efficacy of gintonin in a rabbit model of corneal damage. We investigated the signal transduction pathway of gintonin in human corneal epithelium (HCE) cells to elucidate the underlying molecular mechanism. We next evaluated the therapeutic effects of gintonin, using a rabbit model of corneal damage, by histochemical analysis. Treatment of gintonin to HCE cells induced transient increases of [Ca²⁺ ]i with concentration-dependent and reversible manners. The gintonin-mediated mobilizations of [Ca²⁺ ]i was attenuated by the LPA1/3 receptor antagonist Ki16425, a phospholipase C inhibitor U73122, an inositol 1,4,5-triphosphate receptor antagonist 2-APB, and an intracellular Ca²⁺ chelator BAPTA-AM. Gintonin facilitated in vitro wound healing in a concentration-dependent manner. When applied as an eye-drop to rabbits with corneal damage, gintonin rapidly promoted the recovery. Histochemical analysis also showed gintonin decreased corneal apoptosis and increased corneal cell proliferation. We demonstrated LPA receptor activation by gintonin is linked to in vitro and in vivo therapeutic effects against corneal damage. Gintonin can be applied as a clinical agent for the rapid healing of corneal damage.
URI
http://pubs.kist.re.kr/handle/123456789/64698
ISSN
1229-845X
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KIST Publication > Article
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