Gintonin, an exogenous ginseng-derived LPA receptor ligand, promotes corneal wound healing
- Gintonin, an exogenous ginseng-derived LPA receptor ligand, promotes corneal wound healing
- 임혜원; 김현중; 김준영; 이병환; 최선혜; 김형춘; 안성엽; 정순욱; 장민희; 조익현; 나승열
- Issue Date
- JOURNAL OF VETERINARY SCIENCE
- VOL 18, NO 3-397
- Ginseng gintonin is an exogenous ligand of lysophosphatidic acid (LPA) receptor. Accumulating evidence shows LPA helps a rapid recovery of corneal damage. The aim of this study was to evaluate the therapeutic efficacy of gintonin in a rabbit model of corneal damage. We investigated the signal transduction pathway of gintonin in human corneal epithelium (HCE) cells to elucidate the underlying molecular mechanism. We next evaluated the therapeutic effects of gintonin, using a rabbit model of corneal damage, by histochemical analysis. Treatment of gintonin to HCE cells induced transient increases of [Ca²⁺ ]i with concentration-dependent and reversible manners. The gintonin-mediated mobilizations of [Ca²⁺ ]i was attenuated by the LPA1/3 receptor antagonist Ki16425, a phospholipase C inhibitor U73122, an inositol 1,4,5-triphosphate receptor antagonist 2-APB, and an intracellular Ca²⁺ chelator BAPTA-AM. Gintonin facilitated in vitro wound healing in a concentration-dependent manner. When applied as an eye-drop to rabbits with corneal damage, gintonin rapidly promoted the recovery. Histochemical analysis also showed gintonin decreased corneal apoptosis and increased corneal cell proliferation. We demonstrated LPA receptor activation by gintonin is linked to in vitro and in vivo therapeutic effects against corneal damage. Gintonin can be applied as a clinical agent for the rapid healing of corneal damage.
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