Vertical nanocolumn-assisted pluripotent stem cell colony formation with minimal cell-penetration

Title
Vertical nanocolumn-assisted pluripotent stem cell colony formation with minimal cell-penetration
Authors
양은경김소연김현주강동희구경희이세영김성민김장환윤명한
Keywords
vertical silicon nanocolumn arrays; mouse induced pluripotent stem cells; spherical colonies; stemness
Issue Date
2016-11
Publisher
Nanoscale
Citation
VOL 8-18097
Abstract
The biological applications of vertical nanostructures mostly rely on their intracellular accessibility through the cellular membrane by promoting cell-to-nanostructure interactions. Herein, we report a seemingly counter-intuitive approach for the spontaneous formation of mouse induced pluripotent stem cell (iPSC)-derived three-dimensional spherical colonies with unlimited self-renewal and differentiation potential. The comprehensive analyses of iPSCs cultured on vertical silicon nanocolumn arrays (vSNAs) with various nanocolumn geometries show reduced cell-to-substrate adhesion and enhanced cell-to-cell interactions under optimized vSNA conditions, successfully accommodating the spontaneous production of iPSC-derived spherical colonies. Remarkably, these colonies which were only minimally penetrated by and thereby easily harvested from wafer-sized vSNAs display a substantial increase in pluripotency marker expression and successfully differentiate into three germ layers. Our vSNAs capable of large-scale fabrication, efficient for spherical colony formation, and reusable for multiple iPSC culture could serve as a broad-impact culture platform for stem cell research.
URI
http://pubs.kist.re.kr/handle/123456789/64706
ISSN
2040-3364
Appears in Collections:
KIST Publication > Article
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