Opto-vTrap, an optogenetic trap for reversible inhibition of vesicular release, synaptic transmission, and behavior

Authors
Won, JounghaPankratov, YuriyJang, Minwoo WendyKim, SunpilJu, Yeon HaLee, SangkyuLee, Seung EunKim, AriePark, SoowonLee, C. JustinHeo, Won Do
Issue Date
2022-02-02
Publisher
CELL PRESS
Citation
NEURON, v.110, no.3, pp.423 - +
Abstract
Spatiotemporal control of brain activity by optogenetics has emerged as an essential tool to study brain function. For silencing brain activity, optogenetic probes, such as halorhodopsin and archaerhodopsin, inhibit transmitter release indirectly by hyperpolarizing membrane potentials. However, these probes cause an undesirable ionic imbalance and rebound spikes. Moreover, they are not applicable to use in non-excitable glial cells. Here we engineered Opto-vTrap, a light-inducible and reversible inhibition system to temporarily trap the transmitter -containing vesicles from exocytotic release. Light activation of Opto-vTrap caused full vesicle clusterization and complete inhibition of exocytosis within 1 min, which recovered within 30 min after light off. We found a significant reduction in synaptic and gliotransmission upon activation of Opto-vTrap in acute brain slices. Opto-vTrap significantly inhibited hippocampus-dependent memory retrieval with full recovery within an hour. We propose Opto-vTrap as a next-generation optogenetic silencer to control brain activity and behavior with minimal confounding effects.
Keywords
HIPPOCAMPAL; FEAR; MEMORY; INACTIVATION; STIMULATION; PERFORMANCE; PROJECTIONS; ACTIVATION; NEURONS; CONTEXT; HIPPOCAMPAL; FEAR; MEMORY; INACTIVATION; STIMULATION; PERFORMANCE; PROJECTIONS; ACTIVATION; NEURONS; CONTEXT; CIBN; CRY2; exocytosis inhibition; lariat; Opto-vTrap; optogenetic inhibition; optogenetics; vesicular release inhibition
ISSN
0896-6273
URI
https://pubs.kist.re.kr/handle/201004/115634
DOI
10.1016/j.neuron.2021.11.003
Appears in Collections:
KIST Article > 2022
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE