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dc.contributor.authorJung, Jaeyong-
dc.contributor.authorBong, Ji-Hong-
dc.contributor.authorKim, Tae-Hun-
dc.contributor.authorSung, Jeong Soo-
dc.contributor.authorLee, Changkyu-
dc.contributor.authorKang, Min-Jung-
dc.contributor.authorKim, Hyun Ok-
dc.contributor.authorShin, Hyun-Jin-
dc.contributor.authorPyun, Jae-Chul-
dc.date.accessioned2024-01-19T13:03:55Z-
dc.date.available2024-01-19T13:03:55Z-
dc.date.created2022-01-25-
dc.date.issued2021-12-
dc.identifier.issn1976-0280-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/115982-
dc.description.abstractSeveral endemic corona viruses (eCoVs) have been reported to be the most common etiologic agents for the seasonal common cold and also cause pneumonia. These eCoVs share extensive sequence homology with SARS-CoV-2, and immune responses to eCoVs can cross-react with SARS-CoV-2 antigens. Based on such cross-reactivity of antigens among eCoVs, the IgG antibodies against the spike protein (SP) of severe acute respiratory syndrome coronavirus (SARS-CoV) were isolated from pig serum using magnetic beads immobilized with SARS-CoV SP and a protein-A column. The selectivity of the isolated antibodies was tested using different types of antigens, such as SARS-CoV-2 nucleoprotein (NP), influenza A virus (Beijing type), influenza B virus (Tokio and Florida types), human hepatitis B virus surface antigen (HBsAg), and bovine serum albumin (BSA). From the selectivity test, the anti-SP antibodies isolated from pig serum had sufficient selectivity to other kinds of viral antigens, and the apparent binding constant of the isolated antibodies was approximately 1.5 x 10(-8) M from the surface plasmon resonance (SPR) measurements. Finally, the isolated anti-SP antibodies were applied to the immunoassay of SP using competitive immunoassay configuration. The feasibility of the detection as well as the quantitative analysis of the SARS-CoV viral culture fluid was determined using four viral culture samples, namely, SARS-CoV, SARS-CoV-2, MERS-CoV, and CoV-229E.-
dc.languageEnglish-
dc.publisherKOREAN BIOCHIP SOCIETY-KBCS-
dc.titleIsolation of Antibodies Against the Spike Protein of SARS-CoV from Pig Serum for Competitive Immunoassay-
dc.title.alternativeIsolation of?Antibodies Against the?Spike Protein of?SARS-CoV from?Pig Serum for?Competitive Immunoassay-
dc.typeArticle-
dc.identifier.doi10.1007/s13206-021-00033-0-
dc.description.journalClass1-
dc.identifier.bibliographicCitationBIOCHIP JOURNAL, v.15, no.4, pp.396 - 405-
dc.citation.titleBIOCHIP JOURNAL-
dc.citation.volume15-
dc.citation.number4-
dc.citation.startPage396-
dc.citation.endPage405-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.description.journalRegisteredClasskci-
dc.identifier.kciidART002785721-
dc.identifier.wosid000690356500001-
dc.identifier.scopusid2-s2.0-85113643040-
dc.relation.journalWebOfScienceCategoryBiochemical Research Methods-
dc.relation.journalWebOfScienceCategoryChemistry, Analytical-
dc.relation.journalWebOfScienceCategoryNanoscience & Nanotechnology-
dc.relation.journalResearchAreaBiochemistry & Molecular Biology-
dc.relation.journalResearchAreaChemistry-
dc.relation.journalResearchAreaScience & Technology - Other Topics-
dc.type.docTypeArticle-
dc.subject.keywordAuthorSARS-CoV spike protein-
dc.subject.keywordAuthorCompetitive immunoassay-
dc.subject.keywordAuthorAntibody isolation-
dc.subject.keywordAuthorPig serum-
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KIST Article > 2021
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