Nasal Turbinate Mesenchymal Stromal Cells Preserve Characteristics of Their Neural Crest Origin and Exert Distinct Paracrine Activity
- Authors
- Kim, Hyun-Jee; Shin, Sungho; Jeong, Seon-Yeong; Lim, Sun-Ung; Lee, Dae-Won; Kwon, Yunhee-Kim; Kang, Jiyeon; Kim, Sung-Won; Jung, Chan-Kwon; Lee, Cheolju; Oh, Il-Hoan
- Issue Date
- 2021-04
- Publisher
- MDPI
- Citation
- JOURNAL OF CLINICAL MEDICINE, v.10, no.8
- Abstract
- The sources of mesenchymal stromal cells (MSCs) for cell therapy trials are expanding, increasing the need for their characterization. Here, we characterized multi-donor, turbinate-derived MSCs (TB-MSCs) that develop from the neural crest, and compared them to bone marrow-derived MSCs (BM-MSCs). TB-MSCs had higher proliferation potential and higher self-renewal of colony forming cells, but lower potential for multi-lineage differentiation than BM-MSCs. TB-MSCs expressed higher levels of neural crest markers and lower levels of pericyte-specific markers. These neural crest-like properties of TB-MSCs were reflected by their propensity to differentiate into neuronal cells and proliferative response to nerve growth factors. Proteomics (LC-MS/MS) analysis revealed a distinct secretome profile of TB-MSCs compared to BM and adipose tissue-derived MSCs, exhibiting enrichments of factors for cell-extracellular matrix interaction and neurogenic signaling. However, TB-MSCs and BM-MSCs exhibited comparable suppressive effects on the allo-immune response and comparable stimulatory effects on hematopoietic stem cell self-renewal. In contrast, TB-MSCs stimulated growth and metastasis of breast cancer cells more than BM-MSCs. Altogether, our multi-donor characterization of TB-MSCs reveals distinct cell autonomous and paracrine properties, reflecting their unique developmental origin. These findings support using TB-MSCs as an alternative source of MSCs with distinct biological characteristics for optimal applications in cell therapy.
- Keywords
- turbinate MSC; neural crest; secretome
- ISSN
- 2077-0383
- URI
- https://pubs.kist.re.kr/handle/201004/117180
- DOI
- 10.3390/jcm10081792
- Appears in Collections:
- KIST Article > 2021
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