Characterization of hydrocoptisonine metabolites in human liver microsomes using a high-resolution quadrupole-orbitrap mass spectrometer

Authors
Choi, Su MinKim, YounahLee, JaeickKim, Ju-HyunLee, TaehoMin, Byung SunKim, Jeong AhLee, Sangkyu
Issue Date
2020-12
Publisher
TAYLOR & FRANCIS LTD
Citation
XENOBIOTICA, v.50, no.12, pp.1423 - 1433
Abstract
Hydrocoptisonine is a new compound that has been isolated from the rhizomes ofCoptis chinensis, which belongs to the Ranunculaceae family of Chinese medicines. Although studies onC. chinensishave been reported, the metabolic pathway of hydrocoptisonine in human liver microsomes (HLMs) remains unelucidated. We identified 13 metabolites in HLMs, including six Phase I metabolites and seven glucuronide conjugates, using a high-resolution quadrupole-orbitrap mass spectrometer. The major metabolic pathway was theO-demethylation and mono-hydroxylation of hydrocoptisonine in HLMs. Notably, M3 metabolite wasO-demethylated in dioxolane structures (cyclohexa-3,5-diene-1,2-dione), which was mediated by cytochrome P450 1A2. The locations of hydroxylation and hydroxyl-glucuronidation were identified by analyzing the signature fragments generated as a result of tandem mass spectrometry, indicating hydroxylation at an aliphatic chain or aromatic ring. We determined whether the hydroxylation and glucuronidation occurred in an aromatic moiety (M5 and M12) or an aliphatic moiety (M6 and M13), respectively, based on signature fragments of the metabolites.
Keywords
IN-VITRO METABOLITES; RAT; BERBERINE; CYPs; hydrocoptisonine; human liver microsomes; O-demethylation; UGT
ISSN
0049-8254
URI
https://pubs.kist.re.kr/handle/201004/117738
DOI
10.1080/00498254.2020.1795304
Appears in Collections:
KIST Article > 2020
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