Interconnectable Dynamic Compression Bioreactors for Combinatorial Screening of Cell Mechanobiology in Three Dimensions

Authors
Seo, JungmokShin, Jung-YounLeijten, JeroenJeon, OjuOzturk, Ayca BalRouwkema, JeroenLi, YuanchengShin, Su RyonHajiali, HadiAlsberg, EbenKhademhosseini, Ali
Issue Date
2018-04-25
Publisher
American Chemical Society
Citation
ACS Applied Materials & Interfaces, v.10, no.16, pp.13293 - 13303
Abstract
Biophysical cues can potently direct a cell's or tissue's behavior. Cells interpret their biophysical surroundings, such as matrix stiffness or dynamic mechanical stimulation, through mechanotransduction. However, our understanding of the various aspects of mechanotransduction has been limited by the lack of proper analysis platforms capable of screening three-dimensional (3D) cellular behaviors in response to biophysical cues. Here, we developed a dynamic compression bioreactor to study the combinational effects of biomaterial composition and dynamic mechanical compression on cellular behavior in 3D hydrogels. The bioreactor contained multiple actuating posts that could apply cyclic compressive strains ranging from 0 to 42% to arrays of cell-encapsulated hydrogels. The bioreactor could be interconnected with other compressive bioreactors, which enabled the combinatorial screenings of 3D cellular behaviors simultaneously. As an application of the screening platform, cell spreading, and osteogenic differentiation of human mesenchymal stem cells (hMSCs) were characterized in 3D gelatin methacryloyl (GelMA) hydrogels. Increasing hydrogel concentration from S to 10% restricted the cell spreading, however, dynamic compressive strain increased cell spreading. Osteogenic differentiation of hMSCs was also affected by dynamic compressive strains. hMSCs in 5% GelMA hydrogel were more sensitive to strains, and the 42% strain group showed a significant increase in osteogenic differentiation compared to other groups. The interconnectable dynamic compression bioreactor provides an efficient way to study the interactions of cells and their physical microenvironments in three dimensions.
Keywords
EMBRYONIC STEM-CELLS; BONE-MARROW; MECHANICAL STIMULATION; OSTEOGENIC DIFFERENTIATION; BIOMATERIAL MICROARRAYS; STRAIN; BEHAVIOR; DESIGN; GROWTH; MICROENVIRONMENTS; EMBRYONIC STEM-CELLS; BONE-MARROW; MECHANICAL STIMULATION; OSTEOGENIC DIFFERENTIATION; BIOMATERIAL MICROARRAYS; STRAIN; BEHAVIOR; DESIGN; GROWTH; MICROENVIRONMENTS; high-throughput screening; human mesenchymal stem cells; dynamic compression bioreactor; 3D mechanobiology; mechanical stimulation
ISSN
1944-8244
URI
https://pubs.kist.re.kr/handle/201004/121458
DOI
10.1021/acsami.7b17991
Appears in Collections:
KIST Article > 2018
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE