Disinfection of various bacterial pathogens using novel silver nanoparticle-decorated magnetic hybrid colloids

Abstract

Silver nanoparticles (AgNPs) have long been considered a powerful disinfectant for controlling pathogenic microorganisms. However, AgNPs might have adverse effects on both human health and our ecosystems due to their potential cytotoxicity and the difficulty in recovering them after their release into the environment. In this study, we characterized the antimicrobial efficacy caused by a novel micrometer-sized magnetic hybrid colloid (MHC) containing 7, 15, or 30 nm sized monodispersed AgNPs (AgNP-MHCs), which can be re-collected from the environment using simple procedures, such as a magnet or centrifugation. We evaluated the antibacterial capabilities of AgNP-MHCs against target bacteria (Legionellu pneumuphila, Bacillus subtilis, Escherichia coli, and Clostridium perfringens) and compared them with the inactivation efficacy of AgNPs similar to 30 nm in diameter (nAg30s). Among the different AgNP-MHCs composites evaluated, Ag30-MHCs had the greatest antibacterial effect After 1 h of exposure, more than a 4-logio reduction of L imeumophila and 6-log(10) reduction of B. sublilis was achieved by 4.6 x 10(9) particleslifiL of Ag30-MHCs and Ag30-MHC-Ls. In addition, Ag30-MHC-Ls maintained their strong antibacterial capabilities under anaerobic conditions. Our results indicate that AgNP-MHCs can be considered excellent tools for controlling waterborne bacterial pathogens, with a minimal risk of release into the environment. (C) 2017 Elsevier B.V. All rights reserved.