Detection of Avian Influenza Virus from Cloacal Swabs Using a Disposable Well Gate FET Sensor

Authors
Park, SungwookChoi, JaebinJeun, MinhongKim, YongdeokYuk, Seong-SuKim, Sang KyungSong, Chang-SeonLee, SeokLee, Kwan Hyi
Issue Date
2017-07-05
Publisher
WILEY
Citation
ADVANCED HEALTHCARE MATERIALS, v.6, no.13
Abstract
Current methods to detect avian influenza viruses (AIV) are time consuming and lo inw sensitivity, necessitating a faster and more sensitive sensor for on-site epidemic detection in poultry farms and urban population centers. This study reports a field effect transistor (FET) based AIV sensor that detects nucleoproteins (NP) within 30 minutes, down to an LOD of 10(3) EID50 mL(-1) from a live animal cloacal swab. Previously reported FET sensors for AIV detection have not targeted NPs, an internal protein shared across multiple strains, due to the difficulty of field-effect sensing in a highly ionic lysis buffer. The AIV sensor overcomes the sensitivity limit with an FET-based platform enhanced with a disposable well gate (DWG) that is readily replaceable after each measurement. In a single procedure, the virus-containing sample is immersed in a lysis buffer mixture to expose NPs to the DWG surface. In comparison with commercial AIV rapid kits, the AIV sensor is proved to be highly sensitive, fast, and compact, proving its potential effectiveness as a portable biosensor.
Keywords
FIELD-EFFECT-TRANSISTOR; ELECTRICAL DETECTION; SENSITIVE DETECTION; NUCLEOPROTEIN; CHALLENGES; BIOSENSOR; EVOLUTION; DIAGNOSIS; PLATFORM; BLOOD; FIELD-EFFECT-TRANSISTOR; ELECTRICAL DETECTION; SENSITIVE DETECTION; NUCLEOPROTEIN; CHALLENGES; BIOSENSOR; EVOLUTION; DIAGNOSIS; PLATFORM; BLOOD; avian influenza; cloacal swab; disposable well gate; FET sensors; on-site detection
ISSN
2192-2640
URI
https://pubs.kist.re.kr/handle/201004/122540
DOI
10.1002/adhm.201700371
Appears in Collections:
KIST Article > 2017
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