Full metadata record

DC Field Value Language
dc.contributor.authorYoo, Gu-
dc.contributor.authorDilkaute, Carina-
dc.contributor.authorBong, Ji-Hong-
dc.contributor.authorSong, Hyun-Woo-
dc.contributor.authorLee, Misu-
dc.contributor.authorKang, Min-Jung-
dc.contributor.authorJose, Joachim-
dc.contributor.authorPyun, Jae-Chul-
dc.date.accessioned2024-01-20T01:33:03Z-
dc.date.available2024-01-20T01:33:03Z-
dc.date.created2021-09-01-
dc.date.issued2017-05-
dc.identifier.issn0141-0229-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/122787-
dc.description.abstractThe objective of this study was to present an immunoassay for the diaghosis of Sjogren&apos;s syndrome based on the auto displayed La/SSB protein on the outer membrane of intact E. coil (strain UT-5600) and LPS-free E. coil (ClearColi (TM)). As the first step, an autodisplay vector (pCK002)was transfected into intact E. coli and LPS-free E. coli for comparison of efficiency of autdisplay of La/SSB. The Maximal level of La/SSB expression was estimated to be similar for LPS-free E. coli and intact E. coli at different optimal induction periods. Intact E. coli was found to grow twofold faster than LPS-free E. coli, and the maximal level of expression for LPS-free E. coil was obtained with a longer induction period. When the zeta potential was measured, both intact E. coli and LPS-free E. coli showed negative values, and the autodisplay of negatively charged La/SSB protein (pI < 7) on the outer membrane of intact E. coil and LPS-free E. coli resulted in a slight change in zeta potential values. E. coli with autodisplayed La/SSB protein was used for an immunoassay of anti-La/SSB antibodies for the diagnosis of Sjogren&apos;s syndrome. The surface of E. coli with the autodisplayed antigen was modified with rabbit serum and papain to prevent false positive signals because of nonspecific binding of unrelated antibodies from human serum. LPS-free E. coli With autodisplayed La/SSB protein yielded sensitivity and selectivity of 81.6% and 78.6%, respectively. The Bland-Altman test showed that the immunoassays based on LPS-free E. coli and intact E. coli with autodisplayed La/SSB protein were statistically equivalent to a clinical immunoassay for detection of anti-La/SSB antibodies (confidence coefficient 95%). (C) 2017 Elsevier Inc. All rights reserved.-
dc.languageEnglish-
dc.publisherELSEVIER SCIENCE INC-
dc.subjectZ-DOMAINS-
dc.subjectSURFACE DISPLAY-
dc.subjectE. COLI-
dc.subjectOUTER-MEMBRANE-
dc.subjectBACTERIAL LIPOPOLYSACCHARIDES-
dc.subjectRECOMBINANT PROTEINS-
dc.subjectMEDICAL DIAGNOSIS-
dc.subjectSPR BIOSENSOR-
dc.subjectHUMAN SERUM-
dc.subjectIMMUNOASSAY-
dc.titleAutodisplay of the LaiSSB protein on LPS-free E-coli for the diagnosis of Sjogren&apos;s syndrome-
dc.typeArticle-
dc.identifier.doi10.1016/j.enzmictec.2017.01.007-
dc.description.journalClass1-
dc.identifier.bibliographicCitationENZYME AND MICROBIAL TECHNOLOGY, v.100, pp.1 - 10-
dc.citation.titleENZYME AND MICROBIAL TECHNOLOGY-
dc.citation.volume100-
dc.citation.startPage1-
dc.citation.endPage10-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000396955200001-
dc.identifier.scopusid2-s2.0-85011632622-
dc.relation.journalWebOfScienceCategoryBiotechnology & Applied Microbiology-
dc.relation.journalResearchAreaBiotechnology & Applied Microbiology-
dc.type.docTypeArticle-
dc.subject.keywordPlusZ-DOMAINS-
dc.subject.keywordPlusSURFACE DISPLAY-
dc.subject.keywordPlusE. COLI-
dc.subject.keywordPlusOUTER-MEMBRANE-
dc.subject.keywordPlusBACTERIAL LIPOPOLYSACCHARIDES-
dc.subject.keywordPlusRECOMBINANT PROTEINS-
dc.subject.keywordPlusMEDICAL DIAGNOSIS-
dc.subject.keywordPlusSPR BIOSENSOR-
dc.subject.keywordPlusHUMAN SERUM-
dc.subject.keywordPlusIMMUNOASSAY-
dc.subject.keywordAuthorLPS-free E. coli-
dc.subject.keywordAuthorLipopolysaccharide-
dc.subject.keywordAuthorAutodisplay-
dc.subject.keywordAuthorLa/SSB-
dc.subject.keywordAuthorSjogren&apos-
dc.subject.keywordAuthors syndrome-
Appears in Collections:
KIST Article > 2017
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE