Electrical signaling of enzyme-linked immunosorbent assays with an ion-sensitive field-effect transistor

Authors
Jang, Hyun-JuneAhn, JunhyoungKim, Min-GonShin, Yong-BeomJeun, MinhongCho, Won-JuLee, Kwan Hyi
Issue Date
2015-02-15
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Citation
BIOSENSORS & BIOELECTRONICS, v.64, pp.318 - 323
Abstract
Optical laboratory-based immunoassays, such as enzyme-linked immunosorbent assay (ELISA) give a high sensitivity and specificity of various fatal diseases. However, these assays are no longer efficient in on-spot diagnostics of wide-spreading and contagious infections. At this point in time, portable and handhold devices play a pivotal role in infectious diseases with quick diagnostics at or near the site of the disease propagation. In this paper, we demonstrated a novel electrical immunoassay of ELISA that was not based on optical signaling but on electrical signaling. This was done by combining an ion-sensitive field-effect transistor (ISFET) with ELISA. By harnessing the catalytic reaction of alkaline phosphatase that precipitated silver particles, we effectively overcame the chronic Debye screening length issue of the ISFET. Ultimately, small signal ranging from 1 pg/mL to 10 ng/mL was immensely amplified with the ALP label, regardless of buffer conditions. The sensor platform herein surpassed a sensing capability of conventional ELISA that is considered to have a LOD on the order of similar to 1 ng/mL. The results were compared with those of horseradish peroxidase label, which is generally used for optical analyses in ELISA. Our newly developed ISFET-based portable sensor holds a large potential for point-of-care tools in a variety of diseases, without being limited by the need for expensive equipment such as spectro-photometers. (C) 2014 Elsevier B.V. All rights reserved.
Keywords
SILVER NANOPARTICLES; AMYLOID-BETA; HUMAN SERUM; THIN-FILM; IMMUNOSENSOR; ENHANCEMENT; PERFORMANCE; BIOMARKERS; BIOSENSOR; GRAPHENE; SILVER NANOPARTICLES; AMYLOID-BETA; HUMAN SERUM; THIN-FILM; IMMUNOSENSOR; ENHANCEMENT; PERFORMANCE; BIOMARKERS; BIOSENSOR; GRAPHENE; Immunosensor; Point-of-care device; ISFET; ELISA; ALP
ISSN
0956-5663
URI
https://pubs.kist.re.kr/handle/201004/125762
DOI
10.1016/j.bios.2014.09.020
Appears in Collections:
KIST Article > 2015
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE