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dc.contributor.authorHarayama, Nobuya-
dc.contributor.authorKayano, Tomohiko-
dc.contributor.authorMoriya, Taiki-
dc.contributor.authorKitamura, Naoki-
dc.contributor.authorShibuya, Izumi-
dc.contributor.authorTanaka-Yamamoto, Keiko-
dc.contributor.authorUezono, Yasuhito-
dc.contributor.authorUeta, Yoichi-
dc.contributor.authorSata, Takeyoshi-
dc.date.accessioned2024-01-20T08:04:05Z-
dc.date.available2024-01-20T08:04:05Z-
dc.date.created2021-09-02-
dc.date.issued2014-12-03-
dc.identifier.issn0006-8993-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/126005-
dc.description.abstractWhile magnocellular neurons in the supraoptic nucleus (SON) possess rich Gi/o-mediated mechanisms, molecular and cellular properties of G-protein-activated inwardly rectifying K+ (GIRK) channels have been controversial. Here, properties of GIRK channels are examined by RT-PCR and whole-cell patch-clamp techniques in rat SON neurons. Patch clamp experiments showed that the selective GABA(B) agonist, baclofen, enhanced currents in a high K+ condition. The baclofen-enhanced currents exhibited evident inward rectification and were blocked by the selective GABA(B) antagonist, CGP55845A, the IRK channel blocker, Ba2+, and the selective GIRK channel blocker, tertiapin, indicating that baclofen activates GIRK channels via GABA(B) receptors. The GIRK currents were abolished by N-ethylmaleimide pretreatment, and prolonged by GTP gamma S inclusion in the patch pipette, suggesting that Gi/o proteins are involved. RT-PCR analysis revealed mRNAs for all four GIRK 1-4 channels and for both GABA(B)R1 and GABA(B)R2 receptors in rat SON. However, the concentration-dependency of the baclofen-induced activation of GIRK currents had an EC50 of 110 mu M, which is about 100 times higher than that of baclofen-induced inhibition of voltage-dependent Ca2+ channels. Moreover, baclofen caused no significant changes in the membrane potential and the firing rate. These results suggest that although GIRK channels can be activated by GABA(B) receptors via the Gi/o pathway, this occurs at high agonist concentrations, and thus may not be a physiological mechanism regulating the function of SON neurons. This property that the membrane potential receives little influence from GIRK currents seems to be uncommon for CNS neurons possessing rich Gi/o-coupled receptors, and could be a special feature of rat SON neurons. (C) 2014 Elsevier B.V. All rights reserved.-
dc.languageEnglish-
dc.publisherELSEVIER SCIENCE BV-
dc.subjectMAGNOCELLULAR NEUROSECRETORY-CELLS-
dc.subjectPATCH-CLAMP ANALYSIS-
dc.subjectPOTASSIUM CURRENT-
dc.subjectMEDIATED INHIBITION-
dc.subjectVASOPRESSIN NEURONS-
dc.subjectCALCIUM-CHANNELS-
dc.subjectMESSENGER-RNAS-
dc.subjectNUCLEUS-
dc.subjectMODULATION-
dc.subjectDENDRITES-
dc.titleAnalysis of G-protein-activated inward rectifying K+ (GIRK) channel currents upon GABA(B) receptor activation in rat supraoptic neurons-
dc.typeArticle-
dc.identifier.doi10.1016/j.brainres.2014.10.022-
dc.description.journalClass1-
dc.identifier.bibliographicCitationBRAIN RESEARCH, v.1591, pp.1 - 13-
dc.citation.titleBRAIN RESEARCH-
dc.citation.volume1591-
dc.citation.startPage1-
dc.citation.endPage13-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000345728300001-
dc.identifier.scopusid2-s2.0-84922583869-
dc.relation.journalWebOfScienceCategoryNeurosciences-
dc.relation.journalResearchAreaNeurosciences & Neurology-
dc.type.docTypeArticle-
dc.subject.keywordPlusMAGNOCELLULAR NEUROSECRETORY-CELLS-
dc.subject.keywordPlusPATCH-CLAMP ANALYSIS-
dc.subject.keywordPlusPOTASSIUM CURRENT-
dc.subject.keywordPlusMEDIATED INHIBITION-
dc.subject.keywordPlusVASOPRESSIN NEURONS-
dc.subject.keywordPlusCALCIUM-CHANNELS-
dc.subject.keywordPlusMESSENGER-RNAS-
dc.subject.keywordPlusNUCLEUS-
dc.subject.keywordPlusMODULATION-
dc.subject.keywordPlusDENDRITES-
dc.subject.keywordAuthorSupraoptic nucleus-
dc.subject.keywordAuthorGIRK channels-
dc.subject.keywordAuthorGABA(B) receptors-
dc.subject.keywordAuthorGi/o-
dc.subject.keywordAuthorPatch clamp-
dc.subject.keywordAuthorRT-PCR-
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