Analysis of microRNA and mRNA expression profiles highlights alterations in modulation of the MAPK pathway under octanal exposure

Authors
Song, Mi-KyungChoi, Han-SeamLee, Hyo-SunKim, Youn-JungPark, Yong-KeunRyu, Jae-Chun
Issue Date
2014-01
Publisher
ELSEVIER SCIENCE BV
Citation
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, v.37, no.1, pp.84 - 94
Abstract
Previous environmental microRNA (miRNA) studies have investigated a limited number of candidate miRNAs and have not evaluated functional effects on gene expression. In this study, we aimed to identify octanal (OC)-sensitive miRNAs and to characterize the relationships between miRNAs and expression of candidate genes involved in OC-induced toxicity. Microarray analysis identified 15 miRNAs that were differentially expressed in OC-exposed A549 human alveolar cells. Integrated analyses of miRNA and mRNA expression profiles identified significant miRNA-mRNA anti-correlations. GO analysis of 101 putative target genes showed that the biological category 'MAPK signaling pathway' was prominently annotated. Moreover, we detected increased phosphorylation of p38 MAPK in the OC-exposed group. By integrating the transcriptome and microRNAome, we provide evidence that OC can affect MAPK-induced toxicity signaling. Therefore, this study demonstrates the added value of an integrated miRNA-mRNA approach for identifying molecular events induced by environmental pollutants in an in vitro human model (C) 2013 Elsevier B.V. All rights reserved.
Keywords
GENE-EXPRESSION; MAMMALIAN MICRORNAS; RAT-LIVER; FORMALDEHYDE; KINASE; INDUCTION; CANCER; P38; INFLAMMATION; ACTIVATION; GENE-EXPRESSION; MAMMALIAN MICRORNAS; RAT-LIVER; FORMALDEHYDE; KINASE; INDUCTION; CANCER; P38; INFLAMMATION; ACTIVATION; MicroRNA; Target mRNAs; Aldehydes; Octanal; Mitogen-activated protein kinase; Microarray
ISSN
1382-6689
URI
https://pubs.kist.re.kr/handle/201004/127307
DOI
10.1016/j.etap.2013.11.005
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KIST Article > 2014
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