Full metadata record

DC Field Value Language
dc.contributor.authorSong, Eun Joo-
dc.contributor.authorWerner, Shannon L.-
dc.contributor.authorNeubauer, Jakob-
dc.contributor.authorStegmeier, Frank-
dc.contributor.authorAspden, Julie-
dc.contributor.authorRio, Donald-
dc.contributor.authorHarper, J. Wade-
dc.contributor.authorElledge, Stephen J.-
dc.contributor.authorKirschner, Marc W.-
dc.contributor.authorRape, Michael-
dc.date.accessioned2024-01-20T19:01:36Z-
dc.date.available2024-01-20T19:01:36Z-
dc.date.created2021-09-02-
dc.date.issued2010-07-01-
dc.identifier.issn0890-9369-
dc.identifier.urihttps://pubs.kist.re.kr/handle/201004/131268-
dc.description.abstractThe spliceosome, a dynamic assembly of proteins and RNAs, catalyzes the excision of intron sequences from nascent mRNAs. Recent work has suggested that the activity and composition of the spliceosome are regulated by ubiquitination, but the underlying mechanisms have not been elucidated. Here, we report that the spliceosomal Prp19 complex modifies Prp3, a component of the U4 snRNP, with nonproteolytic K63-linked ubiquitin chains. The K63-linked chains increase the affinity of Prp3 for the U5 snRNP component Prp8, thereby allowing for the stabilization of the U4/U6.U5 snRNP. Prp3 is deubiquitinated by Usp4 and its substrate targeting factor, the U4/U6 recycling protein Sart3, which likely facilitates ejection of U4 proteins from the spliceosome during maturation of its active site. Loss of Usp4 in cells interferes with the accumulation of correctly spliced mRNAs, including those for alpha-tubulin and Bub1, and impairs cell cycle progression. We propose that the reversible ubiquitination of spliceosomal proteins, such as Prp3, guides rearrangements in the composition of the spliceosome at distinct steps of the splicing reaction.-
dc.languageEnglish-
dc.publisherCOLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT-
dc.subjectANAPHASE-PROMOTING COMPLEX-
dc.subjectRECYCLING FACTOR-
dc.subjectFACTOR P110-
dc.subjectU-BOX-
dc.subjectRNA-
dc.subjectPROTEIN-
dc.subjectDOMAIN-
dc.subjectPATHWAY-
dc.subjectDISEASE-
dc.subjectLIGASES-
dc.titleThe Prp19 complex and the Usp4(Sart3) deubiquitinating enzyme control reversible ubiquitination at the spliceosome-
dc.typeArticle-
dc.identifier.doi10.1101/gad.1925010-
dc.description.journalClass1-
dc.identifier.bibliographicCitationGENES & DEVELOPMENT, v.24, no.13, pp.1434 - 1447-
dc.citation.titleGENES & DEVELOPMENT-
dc.citation.volume24-
dc.citation.number13-
dc.citation.startPage1434-
dc.citation.endPage1447-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.identifier.wosid000279405000011-
dc.identifier.scopusid2-s2.0-77954523079-
dc.relation.journalWebOfScienceCategoryCell Biology-
dc.relation.journalWebOfScienceCategoryDevelopmental Biology-
dc.relation.journalWebOfScienceCategoryGenetics & Heredity-
dc.relation.journalResearchAreaCell Biology-
dc.relation.journalResearchAreaDevelopmental Biology-
dc.relation.journalResearchAreaGenetics & Heredity-
dc.type.docTypeArticle-
dc.subject.keywordPlusANAPHASE-PROMOTING COMPLEX-
dc.subject.keywordPlusRECYCLING FACTOR-
dc.subject.keywordPlusFACTOR P110-
dc.subject.keywordPlusU-BOX-
dc.subject.keywordPlusRNA-
dc.subject.keywordPlusPROTEIN-
dc.subject.keywordPlusDOMAIN-
dc.subject.keywordPlusPATHWAY-
dc.subject.keywordPlusDISEASE-
dc.subject.keywordPlusLIGASES-
dc.subject.keywordAuthorUbiquitin-
dc.subject.keywordAuthorK63-linked ubiquitin chains-
dc.subject.keywordAuthorsplicing-
dc.subject.keywordAuthorPrp19 complex-
dc.subject.keywordAuthorUsp4-
Appears in Collections:
KIST Article > 2010
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE