대장균에서 단백질 분비에 대한 Agarivorans albus YKW-34의 Agarase 시그널펩티드의 효과

Abstract

To overcome the limitation of E. coli expression system such as inclusion body formation and disulfide bond failure, we tried to express the heterologous protein as a secreted form. We adopted agarase signal peptide (ASP; 23 amino acid residues) from Agarivorans albus YKW-34 which is one of marine bacteia. When we used ASP to express β-agarase, about 42% activity was detected in media.