Structure-based identification of a novel NTPase from methanococcus jannaschii

Title
Structure-based identification of a novel NTPase from methanococcus jannaschii
Authors
황광연정지형김성호한예선조윤제
Keywords
hyperthetical proteins; nucleotide triphosphatase; functional genomics
Issue Date
1999-07
Publisher
Nature structural biology
Citation
VOL 6, NO 7, 691-696
Abstract
Almost half of the entire set of predicted genomic products from Methanococcus jannaschii are classified as functionally unknown hypothetical proteins. We present a structure-based identification of the biochemical function of a protein with an as yet unknown function from a M. jannaschii gene, Mj0226. The crystal structure of Mj0226 protein determined at 2.2 Å resolution reveals that the protein is a homodimer and each monomer folds into an elongated a/b structure of a new fold family. Comparisons of Mj0226 protein with protein structures in the database, however, indicate that one part of the protein is homologous to some of the nucleotide-binding proteins. Biochemical analysis shows that Mj0226 protein is a novel nucleotide triphosphatase that can efficiently hydrolyze nonstandard nucleotides such as XTP to XMP or ITP to IMP, but not the standard nucleotides, in the presence of Mg2+ or Mn2+ ions.
URI
http://pubs.kist.re.kr/handle/201004/13149
ISSN
1072-8368
Appears in Collections:
KIST Publication > Article
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