Galactosylated Chitosan-g-PEI/DNA Complexes-loaded Poly(organophosphazene) Hydrogel as a Hepatocyte Targeting Gene Delivery System

Authors
Jiang, Hu-LinKim, You-KyoungLee, Sun-MiPark, Mi-RanKim, Eun-MiJin, Yong-MeiArote, RohidasJeong, Hwan-JeongSong, Soo-ChangCho, Myung-HaingCho, Chong-Su
Issue Date
2010-04
Publisher
PHARMACEUTICAL SOC KOREA
Citation
ARCHIVES OF PHARMACAL RESEARCH, v.33, no.4, pp.551 - 556
Abstract
Hydrogels are widely used in drug delivery systems because they can control the release and thereby enhance the efficiency of locally delivered bioactive molecules such as therapeutic drugs, proteins, or genes. For gene delivery, localized release of plasmid DNA or polymer/DNA complexes can transfect cells and produce sustained protein production. We tested the galactosylated chitosan-graft-polyethylenimine (GC-g-PEI)/DNA complexes-loaded poly(organophosphazene) thermosensitive biodegradable hydrogel as a hepatocyte targeting gene delivery system. The poly(organophosphazene) hydrogel loaded with GC-g-PEI/DNA complexes showed low cytotoxicity and higher transfection efficiency than PEI/DNA complexes, as well as good hepatocyte specificity in vitro and in vivo. Our results indicate that poly(organophosphazene) hydrogels loaded with GC-g-PEI/DNA complexes may be a safe and efficient hepatocyte targeting gene delivery system.
Keywords
DNA DELIVERY; IN-VITRO; BIOMEDICAL APPLICATIONS; GRAFT-POLYETHYLENIMINE; SCAFFOLDS; CARRIER; POLYPHOSPHAZENES; MATRICES; RELEASE; DNA DELIVERY; IN-VITRO; BIOMEDICAL APPLICATIONS; GRAFT-POLYETHYLENIMINE; SCAFFOLDS; CARRIER; POLYPHOSPHAZENES; MATRICES; RELEASE; Gene therapy; Galactosylated chitosan-g-polyethylenimine; Poly(organophosphazene) hydrogel; Hepatocyte targeting; Cytotoxicity; Transfection efficiency
ISSN
0253-6269
URI
https://pubs.kist.re.kr/handle/201004/131589
DOI
10.1007/s12272-010-0409-9
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KIST Article > 2010
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