Injectable In Situ-Forming pH/Thermo-Sensitive Hydrogel for Bone Tissue Engineering
- Authors
- Kim, Hea Kyung; Shim, Woo Sun; Kim, Sung Eun; Lee, Kweon-Haeng; Kang, Eunah; Kim, Jong-Ho; Kim, Kwangmeyung; Kwon, Ick Chan; Lee, Doo Sung
- Issue Date
- 2009-04
- Publisher
- MARY ANN LIEBERT, INC
- Citation
- TISSUE ENGINEERING PART A, v.15, no.4, pp.923 - 933
- Abstract
- We developed a novel pH- and thermo-sensitive hydrogel as a scaffold for autologous bone tissue engineering. We synthesized this polymer by adding pH- sensitive sulfamethazine oligomers (SMOs) to both ends of a thermo-sensitive poly(epsilon-caprolactone-co-lactide)-poly(ethylene glycol)-poly(e-caprolactone-co-lactide) (PCLA-PEG-PCLA) block copolymer, yielding a pH/thermo-sensitive SMO-PCLA-PEG-PCLA-SMO block copolymer. The synthesized block copolymer solution rapidly formed a stable gel under physiological conditions (pH 7.4 and 37 degrees C), whereas it formed a sol at pH 8.0 and 37 degrees C, making it injectable. This pH/thermo-sensitive hydrogel exhibited high biocompatibility in a Dulbecco's modified Eagle's medium extract test. Under physiological conditions, the hydrogel easily encapsulated human mesenchymal stem cells (hMSCs) and recombinant human bone morphogenetic protein-2 (rhBMP-2), with encapsulating efficiencies of about 90% and 85%, respectively. To assay for ectopic bone formation in vivo, we subcutaneously injected a polymer solution containing hMSCs and rhBMP-2 into the back of mice, after which we could observe hMSC differentiation for up to 7 weeks. Histological studies revealed mineralized tissue formation and high levels of alkaline phosphatase activity in the mineralized tissue. Therefore, this pH/thermo-sensitive SMO-PCLA-PEG-PCLA-SMO block copolymer demonstrated potential as an injectable scaffold for bone tissue engineering, with in situ formation capabilities.
- Keywords
- MESENCHYMAL STEM-CELLS; MORPHOGENETIC PROTEIN-2; ADIPOSE-TISSUE; OSTEOGENIC DIFFERENTIATION; DELIVERY; PH; DEGRADATION; SCAFFOLDS; MATRICES; MESENCHYMAL STEM-CELLS; MORPHOGENETIC PROTEIN-2; ADIPOSE-TISSUE; OSTEOGENIC DIFFERENTIATION; DELIVERY; PH; DEGRADATION; SCAFFOLDS; MATRICES
- ISSN
- 1937-3341
- URI
- https://pubs.kist.re.kr/handle/201004/132617
- DOI
- 10.1089/ten.tea.2007.0407
- Appears in Collections:
- KIST Article > 2009
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.