카르복실산이 그라프트된 PLLA 필름과 이중기공 지지체의 표면 특성 및 세포점착 거동

Abstract

Biodegradable poly(L-lactic acid) (PLLA) scaffolds have insufficient initial cell binding sites and limited ability to cell adhesion for in vitro and in vivo tissue culture because of their hydrophobic characteristics. Various efforts are now being attempted to overcome these problems. In this study, the biodegradable nonporous PLLA films and their 3D dual pore scaffolds were fabricated by using the solvent casting method and both gas foaming and phase separation techniques, respectively. The obtained hydrophobic nonporous film and dual pore scaffold surfaces were hydrophilized by using plasma glow discharge treatment and the grafting of carboxyl acid-containing monomers such as acrylic acid (AA), maleic acid (MA), itaconic acid (IA), and trans-aconitic acid (tAA). The surface modification of nonporous films and their dual pore scaffolds was confirmed by water contact angle, electron spectroscopy for chemical analysis (ESCA), and carboxyl group content. The hydrophilized nonporous PLLA films and their dual pore scaffolds which have carboxyl acids on their surface by plasma treatment demonstrated the improved adhesion and proliferation of fibroblasts as compared to the unmodified one.