Determination of vitamin E in porcine plasma after oral administration of vitamin E-enriched feedstuffs using high performance liquid chromatography
- Authors
- Jung, BH; Chang, BS; Chung, BC
- Issue Date
- 2001-11
- Publisher
- MARCEL DEKKER INC
- Citation
- ANALYTICAL LETTERS, v.34, no.13, pp.2303 - 2310
- Abstract
- A simple and reliable, high-performance liquid chromatographic (HPLC) method for the determination of vitamin E in porcine plasma with UV detection is described. Liquid-liquid extraction with ether was used, and the extraction recovery was improved to 82% by adding NaCl to the aqueous phase. The mobile phase was comprised of methanol and water. and a gradient system. was used. in which the percentage of methanol was increased from 70% to 100%. The flow rate was 1.5 ml/min, and the peaks were monitored at 220 nm in a UV detector. The detection limit was 1 mug/ml, and the coefficient of variation was less than 10% in the 3-15 mug/ml range and less than 12.3% in the 1 mug/ml. This method was adapted to the analysis of porcine plasma obtained after oral administration of vitamin E-enriched feedstuffs. After the analysis, it was apparent that the plasma level was maintained within 1-5 mug/ml in all of the pigs.
- Keywords
- ALPHA-TOCOPHEROL; BETA-CAROTENE; RETINOL; INFANTS; ALPHA-TOCOPHEROL; BETA-CAROTENE; RETINOL; INFANTS; vitamin E; HPLC; salting out effect; porcine plasma
- ISSN
- 0003-2719
- URI
- https://pubs.kist.re.kr/handle/201004/140095
- DOI
- 10.1081/AL-100107296
- Appears in Collections:
- KIST Article > 2001
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