Isolation of virus-cell fusion inhibitory components from Eugenia caryophyllata

Abstract

The fractionation of Eugenia caryophyllata (Myrtaceae) guided by the syncytia formation inhibition assay led to the isolation of four tannins (eugeniin, casuarictin. 1.3-di-O-galloyl-4,6-(S)-hexahydroxydiphenoyl-beta -D-glucopyranose and tellimagrandin I), and two chromones (biflorin and isobiflorin). Among the isolated compounds, tellimagrandin (4) showed a significantly high inhibitory activity on the syncytia formation with an IC50 value of 16.12 +/- 1.98 mug/ml.