Determination of derivatized clenbuterol by use of gas chromatography and tandem mass spectrometry on an ion trap using negative ion chemical ionization

Abstract

Doping control studies for the detection of clenbuterol in human urine was studied. Confirmation was achieved by trifluoroacetylation after cyclic fluorophenylboronation to produce FPBA-clenbuterol, for derivatization over 10 min at 80 degrees C, measured using negative chemical ionization (NICl) and NICl-tanden mass spectrometry (MS/MS) on an ion trap mass spectrometer, In the NICl-MS/MS mode, the mit 440 ion was selected as the precursor ion. This ion may be formed from FPBA-clenbuterol trifluoroacetate, which has a molecular weight of 476, having lost HCl, Derivatized clenbuterol was detected to 50 fg/mL, in spiked human urine. We applied the above procedure to two positive samples, one as a proficiency test and the other for reaccreditation, and achieved good results. (C) 1998 John Wiley & Sons, Ltd.