Isolation and identification of carbon monoxide utilizing anaerobe. eubacterium limosum KIST612

Abstract

Carbon monoxide (CO)-utilizing acetogens were enriched and KIST612 isolated from anaerobic digester fluid was selected for its abilities to tolerate high CO and acetate concentration. The isolate KIST612 was identified as Eubacterium limosum based on the morphological and biochemical characteristics, G+C content of DNA and 16S rRNA sequence analysis. E. lunosum KIST612 produced acetate and butyrate from CO. The optimum temperature and pH for the growth and acids formations were 37°C and 7.0, respectively. The growth rate and acids productivity of E. limosum KIST612 were higher than those of any other known acetogens when CO was used as the sole energy and carbon source.