Influence of coating ligands for enzyme-linked immunosorbent assay of toluene

Title
Influence of coating ligands for enzyme-linked immunosorbent assay of toluene
Authors
김소영이남택최명자
Keywords
coating ligand; Competitive ELISA; Toluene; Benzene; Xylene
Issue Date
2001-09
Publisher
Bulletin of the Korean Chemical Society
Citation
VOL 22, NO 9, 953-957
Abstract
The specificity of conjugation site for coating ligands was investigated using toluene-bovine serum albumin (BSA) conjugates in which BSA was conjugated at the position of o-, m-, and r-toluic acid. Toluene-BSA conjugated at r-position showed a binding activity of about 89-95%, whereas, those conjugated at o- and mposition of toluene exhibited a binding activity of 5 and 11%, respectively. On the basis of the above result, coating ligands with various proteins (OVA, BSA, KLH) were compared by conjugating with r-toluic acid, and toluene-KLH was considered as the best coating ligand for this ELISA. Indirect competitive ELISA method was developed using anti-toluene antibody and r-position conjugated toluene-KLH. The doseresponse curve constructed after kinetic and optimization studies showed a 1 ´ 10-4-1 ´ 102 mM detectable response range with 0.1 mM detectability. In specificity test of the antibody, the binding capabilities of aromatic compounds substituted with nitro-, alkyl-, chloro-, and hydroxyl group were larger rather than those of aromatic compounds (benzene, toluene and xylene) themselves. Also, tests with soil and water samples that had been spiked with toluene resulted in 102.7-113.7% recovery.
URI
http://pubs.kist.re.kr/handle/201004/16225
ISSN
0253-2964
Appears in Collections:
KIST Publication > Article
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