Genotoxicity study of sophoricoside, a constituent of sophora japonica, in bacterial and mammalian cell system

Title
Genotoxicity study of sophoricoside, a constituent of sophora japonica, in bacterial and mammalian cell system
Authors
Youn-Jung KimHyo-Joung ParkYoungsoo KimMi-Kyung KimSeung-Ho LeeSang-Hun Jung류재천
Keywords
sophoricoside; bacterial reverse mutation; chromosomal aberration; comet assay
Issue Date
2001-12
Publisher
Environmental Mutagens & Carcinogens (한국환경성돌연변이발암원학회지)
Citation
VOL 21, NO 2, 99-105
Abstract
Sophoricoside was isolated as the inhibitor of IL-5 bioactivity from Sophora japonica (Leguminosae). It has been reported to has an anti-inflammatory effect on rat paw edema model. To develope as an anti-allergic drug, genotoxicity of sophoricoside was investigated in bacterial and mammalian cell system such as Ames bacterial reversion test, chromosomal aberration assay and single cell gel electrophoresis (Comet) assay. As results, in the range of 1,250~40 mg/plate sophoricoside concentrations was not shown significant mutagenic effects in Salmonella typhimurium TA 98, TA 100, TA 1535 and TA 1537 strains in Ames test. The 80% cell growth inhibition concentration (IC80) of sophoricoside was determined as above 5,000 mg/ml in Chinese hamster lung (CHL) fibroblast cell and L5178Y mouse lymphoma cell line for the chromosomal aberration and comet assay, respectively. Sophoricoside was not induced chromosomal aberration in CHL fibroblast cell at concentrations of 700, 350 and 175 mg/ml or 600, 300 and 150 mg/ml in the absence or presence of S-9 metabolic activation system, respectively. Also, in the comet assay, the induction of DNA damage was not observed in L5178Y mouse lymphoma cell line both in the absence or presence of S-9 metabolic activation system. From these results, no genotoxic effects of sophoricoside were observed in bacterial and mammalian cell systems used in these experiments.
URI
http://pubs.kist.re.kr/handle/201004/16739
ISSN
1225-6307
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KIST Publication > Article
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