Genotoxicity study of sophoricoside, a constituent of sophora japonica, in bacterial and mammalian cell system
- Genotoxicity study of sophoricoside, a constituent of sophora japonica, in bacterial and mammalian cell system
- Youn-Jung Kim; Hyo-Joung Park; Youngsoo Kim; Mi-Kyung Kim; Seung-Ho Lee; Sang-Hun Jung; 류재천
- sophoricoside; bacterial reverse mutation; chromosomal aberration; comet assay
- Issue Date
- Environmental Mutagens & Carcinogens (한국환경성돌연변이발암원학회지)
- VOL 21, NO 2, 99-105
- Sophoricoside was isolated as the inhibitor of IL-5 bioactivity from Sophora japonica (Leguminosae).
It has been reported to has an anti-inflammatory effect on rat paw edema model. To develope as an anti-allergic
drug, genotoxicity of sophoricoside was investigated in bacterial and mammalian cell system such as Ames
bacterial reversion test, chromosomal aberration assay and single cell gel electrophoresis (Comet) assay. As
results, in the range of 1,250~40 mg/plate sophoricoside concentrations was not shown significant mutagenic
effects in Salmonella typhimurium TA 98, TA 100, TA 1535 and TA 1537 strains in Ames test. The 80% cell
growth inhibition concentration (IC80) of sophoricoside was determined as above 5,000 mg/ml in Chinese
hamster lung (CHL) fibroblast cell and L5178Y mouse lymphoma cell line for the chromosomal aberration and
comet assay, respectively. Sophoricoside was not induced chromosomal aberration in CHL fibroblast cell at
concentrations of 700, 350 and 175 mg/ml or 600, 300 and 150 mg/ml in the absence or presence of S-9 metabolic
activation system, respectively. Also, in the comet assay, the induction of DNA damage was not observed in
L5178Y mouse lymphoma cell line both in the absence or presence of S-9 metabolic activation system. From
these results, no genotoxic effects of sophoricoside were observed in bacterial and mammalian cell systems used
in these experiments.
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