The crystal structure of flap endonuclease-1 from methanococcus jannaschii
- The crystal structure of flap endonuclease-1 from methanococcus jannaschii
- 황광연; 백규원; 김혜연; 조윤제
- Issue Date
- Nature structural biology
- VOL 5, NO 8, 707-713
- Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication
and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 Å resolution, reveals
an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are
clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole
large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the
nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the
junction between single and double-stranded regions of flap DNA.
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