The crystal structure of flap endonuclease-1 from methanococcus jannaschii

Title
The crystal structure of flap endonuclease-1 from methanococcus jannaschii
Authors
황광연백규원김혜연조윤제
Issue Date
1998-08
Publisher
Nature structural biology
Citation
VOL 5, NO 8, 707-713
Abstract
Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 Å resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.
URI
http://pubs.kist.re.kr/handle/201004/18668
ISSN
1072-8368
Appears in Collections:
KIST Publication > Article
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