Modulation of Cav3.1 T-type Ca2+ channels by the ran binding protein RanBPM

Title
Modulation of Cav3.1 T-type Ca2+ channels by the ran binding protein RanBPM
Authors
김태현김선오윤형문정광철한예선신희섭임혜원
Keywords
T-type Ca2+ channels; Cav3.1; α1G subunit; RanBPM; PKC; *2008년도개인평가에반영되었음
Issue Date
2009-01
Publisher
Biochemical and biophysical research communications
Citation
VOL 378, NO 1, 15-20
Abstract
In order to study the currently unknown cellular signaling pathways of Cav3.1 T-type Ca2+ channels (Cav3.1 channels), we performed a yeast two-hybrid screening using intracellular domains of Cav3.1 a1 subunit as bait. After screening the human brain cDNA library, several proteins, including RanBPM, were identified as interacting with Cav3.1 channels. RanBPM was found to bind to the cytoplasmic intracellular loop between transmembrane domains I and II of Cav3.1 channels. Using whole-cell patch-clamp techniques, we found that Cav3.1 currents were increased by the expression of RanBPM in HEK293/Cav3.1 cells. We next examined whether RanBPM affected the biophysical properties and plasma membrane expression of Cav3.1 channels. Furthermore, we showed that the PKC activator inhibited Cav3.1 currents, an effect that was abolished by the expression of RanBPM. These results suggest that RanBPM could be a key regulator of Cav3.1 channel-mediated signaling pathways.
URI
http://pubs.kist.re.kr/handle/201004/33595
ISSN
1090-2104
Appears in Collections:
KIST Publication > Article
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