Escherichia coli with autodisplayed Z-domain of protein A for signal amplification of SPR biosensor

Title
Escherichia coli with autodisplayed Z-domain of protein A for signal amplification of SPR biosensor
Authors
Joachim Jose정지원Byoung-Jin JeonRuth Maria Maas남창훈변재철
Keywords
Autodisplay; SPR biosensor; Myoglobin; Immunosensor; Signal amplification
Issue Date
2009-01
Publisher
Biosensors & bioelectronics
Citation
VOL 24, NO 5, 1324-1329
Abstract
Generally, an immunoaffinity SPR biosensor detects a target analyte in a sample through highly selective adsorption by using the antigen&#8211;antibody interaction. For improving the sensitivity, various kinds of particles have been added to the already bound analytes on the SPR biosensor (sandwich assay). In this work, signal amplification was demonstrated by the expression of the IgG-binding Z-domain of protein A on the outer membrane of Escherichia coli via “Autodisplay”. The amount of Z-domain of protein A expressed on the outer membrane was calculated to be 280,000 molecules per cell. In addition, the IgGbinding ability of the expressed proteinwas characterized using FACS analysis. The signal amplification of the SPR biosensor was performed in the sandwich assay format using a model of horseradish peroxidase (HRP); the limit of detectionwas determined to be significantly improved from1 g/ml to 1 ng/ml. Finally, myoglobin analysis was demonstrated for the medical diagnosis of cardiac diseases. The detection limit was estimated to be improved from 10 ng/ml to <1 ng/ml. These results showthat Z-domain-displaying E. coli can be successfully used for the signal amplification of immunoaffinity biosensors, thereby improving the sensitivity and the limit of detection.
URI
http://pubs.kist.re.kr/handle/201004/34732
ISSN
0956-5663
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE