Microbial community dynamics in replicate membrane bioreactors - Natural reproducible fluctuations

Title
Microbial community dynamics in replicate membrane bioreactors - Natural reproducible fluctuations
Authors
Michael W. Falk송경근Michael G. MatiasekStefan Wuertz
Keywords
Membrane bioreactor; T-RFLP; Microbial community dynamics; Ammonia oxidizing bacteria; 16S rRNA genes
Issue Date
2009-02
Publisher
Water research
Citation
VOL 43, NO 3, 842-852
Abstract
We operated 4 replicate membrane bioreactors (MBRs) in parallel to test if an acclimated seed inoculum would evolve similarly following even distribution into replicates. A cloning and sequencing library of 16S rRNA genes was obtained from the seed inoculum complemented with terminal restriction fragment length polymorphism (T-RFLP; n ¼ 18 per reactor) analysis over the study period (n ¼ 113 d) that targeted the 16S rRNA gene. The amoA functional gene was also monitored by T-RFLP. The T-RFLP results were analyzed by means of diversity indices, an adaptation of a moving window of similarity approach within each MBR, and non-metric multi-dimensional scaling (NMS) accompanied with multi-response permutation procedures (MRPP) to assess community interrelationships amongst MBRs. Based on the 16S rRNA microbial communities, the 4 MBRs initially diverged away from one another, followed by a convergence on Day 4. From thereon, the 16S rRNA-based communities evolved similarly throughout (average p-value ¼ 0.49 from pair-wise MRPP). In contrast, the nitrifying communities did not undergo any discernable shift over time amongst MBRs according to T-RFLP analysis of amoA and revealed one cluster by NMS (average p-value ¼ 0.83 from pair-wise MRPP). The study demonstrates that acclimated microbial communities evolve similarly over time in engineered systems when operational parameters are left unchanged.
URI
http://pubs.kist.re.kr/handle/201004/34969
ISSN
0043-1354
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