Stretching of Electrokinetically Tethered DNA in Nanochannels

Title
Stretching of Electrokinetically Tethered DNA in Nanochannels
Authors
이병철신현준이상엽
Keywords
Nanochannels; Electrokinetically Tethered DNA; DNA elongation; Relaxation of γ-DNA; DNA optical mapping
Issue Date
2009-04
Publisher
제 11회 한국 MEMS 학술대회 논문집
Citation
, 105-106
Abstract
The simultaneously tethered and stretched behavior of λ-DNA (48.5kbp) in SiO2 nanochannels was investigated upon high electrical fields. The transverse section of nanochannel is a trapezoid having horns on the top side and the dimensions are 200nm in bottom side, 450nm in top side, and 400nm in height. With fields of 20kV/m or above, the DNA traps through the nanochannels and spontaneously extends 92 percent of full DNA length by electrostatic force. After removing the electrical fields, the relaxation of the DNA molecule is measured as 9.9sec. The experiments show the possibility to use this device for DNA optical mapping.
URI
http://pubs.kist.re.kr/handle/201004/35041
Appears in Collections:
KIST Publication > Conference Paper
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