Effect of initial 1,3-propanediol concentration on 1,3-propanediol production by Klebsiella pneumonia

Title
Effect of initial 1,3-propanediol concentration on 1,3-propanediol production by Klebsiella pneumonia
Authors
최훈공성욱오민규상병인엄영순
Keywords
Klebsiella pneumonia DSM 4799; glycerol; 1,3-propanediol
Issue Date
2009-04
Publisher
생물공학회
Abstract
Klebsiella pneumonia DSM 4799 can convert glycerol to 1,3-propanediol, which is a basic ingredient for polytrimethylene terephthalate(PTT), lubricant, solvents, and other end uses. In this study, inhibitory effect of initial concentration of 1,3-propandiol (1,3-PD) on 1,3-PD production was investigated. The initial concentration of 1,3-PD affected the growth and 1,3-PD production of K. pneumonia DSM4799. With glycerol 20 g/L, the growth and the glycerol consumption rate were dramatically decreased with an initial 1,3-PD concentration higher than 40 g/L. When 1,3-PD was added at the initial concentration of 0, 20, 40, and 60 g/L, the 1,3-PD production was 7.9, 5.9, 5.3, and 0 g/L, respectively, after 12 hrs of cultivation. There was little 1,3-PD production with more than 60 g/L 1,3-PD. The production of other products such as 2,3-butanediol, ethanol, and bio-gas decreased as initial 1,3-PD concentration increased. Unlike those products, acetic acid was produced more with 40 and 60 g/L 1,3-PD than with 0 and 20 g/L, implying that inhibited cells might produce more acids due to the change in metabolism.
URI
http://pubs.kist.re.kr/handle/201004/35053
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KIST Publication > Conference Paper
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