Rapid exchange of oil-phase in microencapsulation chip to enhance cell viability
- Rapid exchange of oil-phase in microencapsulation chip to enhance cell viability
- 김충; 이강선; 김영은; 이규정; 이수현; 김태송; 강지윤
- Issue Date
- Lab on a chip
- VOL 9, NO 9, 1294-1297
- This paper describes a microfluidic device for the microencapsulation of cells in alginate beads to
enhance cell viability. The alginate droplet including cells was gelified with calcified oleic acid, using
two-phase microfluidics. The on-chip gelation had generated monodisperse spherical alginate beads,
which could not be readily obtained via conventional external gelation in a calcium chloride bath.
However, the prolonged exposure of encapsulated cells to the toxic oil phase caused serious damage to
the cells. Therefore, we proposed the formulation of a rapid oil-exchange chip which transforms the
toxic oleic acid to harmless mineral oil. The flushing out of oleic acid after the gelation of alginate beads
effected a dramatic increase in the viability of P19 embryonic carcinoma cells, up to 90%. The
experimental results demonstrated that the cell viability was proportional to the flow rate of squeezing
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