In vivo suppression of bisphenol A on estradiol 2- and 4-hydroxylase activities in hepatic microsomal fractions of male and female Sprague-Dawley rats

Title
In vivo suppression of bisphenol A on estradiol 2- and 4-hydroxylase activities in hepatic microsomal fractions of male and female Sprague-Dawley rats
Authors
보야 누그라하윤애린락쉬미조효주정봉철권오승
Keywords
Bisphenol A; Estradiol 2-hydroxylase; Estradiol 4-hydroxylase; Estrogen metabolites; rat
Issue Date
2009-04
Publisher
Biomolecules & Therapeutics
Citation
VOL 17, NO 2, 188-198
Abstract
This work was conducted to investigate the effect of bisphenol A (BPA) on estradiol (E2) 2-and 4-hydroxylase activities in the liver, kidney and lung tissues of male and female rats. After intraperitoneal administration of BPA to male and female rats for 4 days at 0, 10, and 50 mg/kg, the conversion of the substrate for hepatic and extra-hepatic enzyme activities was measured by GC/MSD. The result showed decreases of body and organ weights at 50 mg/kg BPA of male and female rats. Male hepatic E2 2-hydroxylase activity was inhibited by 68% at 10 mg/kg and by 82% at 50 mg/kg BPA. Female hepatic E2 2-hydroxylase activity was decreased by 46% at 10 mg/kg and by 56% at 50 mg/kg to the control. E2 4-hydroxylase was inhibited by 57 and 57% at 10 mg/kg and 54 and 78% at 50 mg/kg in liver of female and male, respectively. The urinary excretion rate of 2-hydroxyestradiol (2-OHE), androsterone and testosterone in urine of female rats with 50 mg/kg BPA were decreased significantly. The results showed that 50 mg/kg BPA was decreased E2 2-and 4-hydroxylase activities in liver, but not in other tissues. The urinary excretion rates of 2-OHE, androsterone and testosterone were also decreased. In liver, estrogenic enzyme activity were higher in male than female. These results suggest that BPA can disrupt estrogen metabolism by suppressing E2 2-and 4-hydroxylase activities in the liver of male and female rats.
URI
http://pubs.kist.re.kr/handle/201004/35215
ISSN
1976-9148
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KIST Publication > Article
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