Rapid microfluidic separation of magnetic beads through dielectrophoresis and magnetophoresis

Title
Rapid microfluidic separation of magnetic beads through dielectrophoresis and magnetophoresis
Authors
제가타김충박현직강지윤김태송김상경
Keywords
Dielectrophoresis; Magnetophoresis; Microfluidics; Superparamagnetic; microbeads
Issue Date
2009-05
Publisher
Electrophoresis
Citation
VOL 30, 1457-1463
Abstract
We present the design and fabrication of a new microfluidic device in which the dielectrophoresis and magnetophoresis phenomena were used for the separation of the superparamagnetic microbeads of different sizes. By exploiting the fact that two different particles can exhibit different dielectrophoretic force–frequency spectra, we utilize this device to perform multiplex detection from a single sample solution. We found the transition frequency range for 1, 2.8, and 4.5 mm magnetic beads using our device. Beadbased analysis revealed that a high separation efficiency ( 90%) could be obtained from a single sample solution containing both 4.5 and 2.8 mm beads. The average flow velocity of the beads was maintained at 9.8 mm/s, enabling fast analysis with a smaller amount of reagents. The magnetic field distribution on the beads and the bead flow at the channel cross section for different dielectrophoretic conditions was obtained using CFD-ACE1 simulation. Issues relating to the fabrication and operation of the device are discussed in detail. Finally, we demonstrated the feasibility of parallel detection/trapping of different beads on the same chip. This separation approach offers the performance of multiplex analysis in lab-on-a-chip devices.
URI
http://pubs.kist.re.kr/handle/201004/35236
ISSN
0173-0835
Appears in Collections:
KIST Publication > Article
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