Quantification of disease marker in undiluted serum using an actuating layer-embedded microcantilever

Title
Quantification of disease marker in undiluted serum using an actuating layer-embedded microcantilever
Authors
황교선전혜경이상명김상경김태송
Issue Date
2009-05
Publisher
Journal of applied physics
Citation
VOL 105, 102017-1-102017-7
Abstract
In this study, we describe the application feasibility of a dynamic microcantilever with regard to the detection of a specific protein in human serum or real blood using an end-point analysis. The mechanical response i.e., resonant frequency of a functionalized dynamic microcantilever was shown to be altered by molecular interactions, which allowed for the detection of biomolecules present in small quantities without any additional signal enhancements, such as labeling. For the application of the microcantilever sensors to bioassays of serum samples, the mechanical response from the nonspecific adsorption of abundant proteins must be reduced, because it significantly influences the output signal deviation of the microcantilever sensor. We implemented a label-free prostate specific antigen PSA detection protocol in standard serum via our established process, which was designed to minimize nonspecific protein adsorption. PSA is a tumor marker for prostate cancer, with a threshold concentration of 2–4 ng/ml 7.2–14.4 pM for the distinction between patients and normal individuals. The dynamic range of our dynamic microcantilever-based PSA assay on the background of standard serum ranged between 0.1 and 100 ng/ml 3.6 and 3600 pM . It was suggested that the dynamic microcantilever might allow for the sensitive label-free detection of disease markers in an actual human sample.
URI
http://pubs.kist.re.kr/handle/201004/35392
ISSN
0021-8979
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KIST Publication > Article
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