Quantification of disease marker in undiluted serum using an actuating layer-embedded microcantilever
- Quantification of disease marker in undiluted serum using an actuating layer-embedded microcantilever
- 황교선; 전혜경; 이상명; 김상경; 김태송
- Issue Date
- Journal of applied physics
- VOL 105, 102017-1-102017-7
- In this study, we describe the application feasibility of a dynamic microcantilever with regard to the
detection of a specific protein in human serum or real blood using an end-point analysis. The
mechanical response i.e., resonant frequency of a functionalized dynamic microcantilever was
shown to be altered by molecular interactions, which allowed for the detection of biomolecules
present in small quantities without any additional signal enhancements, such as labeling. For the
application of the microcantilever sensors to bioassays of serum samples, the mechanical response
from the nonspecific adsorption of abundant proteins must be reduced, because it significantly
influences the output signal deviation of the microcantilever sensor. We implemented a label-free
prostate specific antigen PSA detection protocol in standard serum via our established process,
which was designed to minimize nonspecific protein adsorption. PSA is a tumor marker for prostate
cancer, with a threshold concentration of 2–4 ng/ml 7.2–14.4 pM for the distinction between
patients and normal individuals. The dynamic range of our dynamic microcantilever-based PSA
assay on the background of standard serum ranged between 0.1 and 100 ng/ml 3.6 and 3600 pM .
It was suggested that the dynamic microcantilever might allow for the sensitive label-free detection
of disease markers in an actual human sample.
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