Specific Interaction of Cells with Biodegradable Polymers Containing Some Peptide Ligands for Biomedical Applications

Title
Specific Interaction of Cells with Biodegradable Polymers Containing Some Peptide Ligands for Biomedical Applications
Authors
한동근김수형박철호박귀덕
Keywords
Peptide; Cell; Biodegradable Polymers; Ligand
Issue Date
2009-06
Publisher
2nd Asian Biomaterials Congress
Abstract
Some synthetic bioactive peptides play a role to interact specifically with appropriate cells in biomedical applications for tissue engineering and coronary stents. Recently, the peptide ligands such as Arg-Gly-Asp (RGD), Arg-Glu-Asp-Val (REDV), and Tyr-Ile-Gly-Ser-Arg (YIGSR) have been immobilized on polymeric materials to improve specific biological activity. In this study, the biodegradable and biocompatible polymers were prepared by UV polymerization and the terminal groups in the polymers were activated and then the peptide, GRGD and GREDV were grafted to promote specific cell adhesion. Surface and bulk characteristics of the obtained peptide-grafted polymers were confirmed from ATR-FTIR, ESCA, time-of-flight secondary ion mass spectrometer (TOF-SIMS), immobilized peptide amount, water contact angle, and biodegradation test. From the result of cell culture, the adhesions of fibroblasts to GRGD-modified polymer and human umbilical vein endothelial cells (HUVECs) to GREDV-modified polymer produced the high amount of extracellular matrix (ECM), respectively. In addition, SEM and H&E staining demonstrated that GRGD and GREDV have ability to promote specific cell adhesion. The prepared biocompatible polymers have good mechanical strength, biodegradation property, and ability to promote specific cell adhesion by immobilization of bioactive ligands such as RGD and REDV. Based on the results, peptide immobilized polymers are expected to be applicable as scaffolds for tissue regeneration and vascular stents for the induction of reendothelization.
URI
http://pubs.kist.re.kr/handle/201004/35468
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KIST Publication > Conference Paper
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