ELECTRICAL IMMOBILIZATION AND SPONTANEOUS STRETCHING OF λ-PHAGE DNA IN NANOCHANNELS

Title
ELECTRICAL IMMOBILIZATION AND SPONTANEOUS STRETCHING OF λ-PHAGE DNA IN NANOCHANNELS
Authors
이상엽이병철강찬민김진식강지윤신현준
Keywords
Optical DNA Mapping; Nanochannel; DNA Manipulation; DNA Stretching; DNA Tethering
Issue Date
2009-06
Publisher
2009 Gordon Research Conference
Abstract
Electrical immobilization of double stranded lambda DNA and its stretching has been investigated in Si nanochannels. DNA has been stained with intercalating TOTO-1, but no other markers or functional modification has been added or made to DNA. DNA began to show the immobilization behavior at relatively high electrical field above 20kV/m while DNA showed fluent movement along the nanochannels at low electrical field. DNA Showed spontaneous elongations as soon as it is immobilized. DNA was stretched up to about 90 percent of its contour length.
URI
http://pubs.kist.re.kr/handle/201004/35494
Appears in Collections:
KIST Publication > Conference Paper
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