Inhibitory Effects of Baicalin from Scutellaria Baicalensis on Oxidative Stress Induced Retinal Ganglion Cell Death

Title
Inhibitory Effects of Baicalin from Scutellaria Baicalensis on Oxidative Stress Induced Retinal Ganglion Cell Death
Authors
정상훈Neville N. Osborne
Keywords
baicalin; Scutellaria baicalensis; RGC-5; neuroprotection; oxidative stress
Issue Date
2009-06
Publisher
The American Society of Pharmacognosy
Abstract
This study was to determine whether baicalin from Scutellaria baicalensis could attenuate oxidative stress induced a transformed retinal ganglion cells (RGC-5 cells) death. RGC-5 cells in culture were given one of three different insults, light (1000 lux for 48 h), hydrogen peroxide (200 µM H2O2 for 24 h) or serum deprivation (48 h), after which cell survival and reactive oxygen species (ROS) formation were assayed. Moreover, a lipid peroxidation assay was used to compare the antioxidant capacity of baicalin with the flavonoid, epigallocatechin gallate (EGCG). Baicalin significantly attenuated the negative insult of light, H2O2 and serum withdrawal to RGC -5 cells. In the lipid peroxidation studies, baicalin was also found to be equally effective as EGCG as an antioxidant. Moreover, baicalin attenuated ROS production caused by three different insults by using the ROS-sensitive probe, dihydroethidium (DHE). Baicalin also inhibited the apoptosis by use of the APOPercentageTM kit. In conclusion, based on these data, we suggest that the effect of baicalin in attenuating three different models of oxidative stress induced cell viability is due to potent antioxidative as well as anti-apoptosis activities.
URI
http://pubs.kist.re.kr/handle/201004/35560
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KIST Publication > Conference Paper
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