Detection of Hepatitis B Virus (HBV) DNA at femtomolar concentrations using a silica nanoparticle-enhanced microcantilever sensor
- Detection of Hepatitis B Virus (HBV) DNA at femtomolar concentrations using a silica nanoparticle-enhanced microcantilever sensor
- 차병학; 이상명; 박재찬; 황교선; 김상경; 이윤식; 주병권; 김태송
- Dynamic microcantilever; HBV DNA; Sensitivity enhancement; Sandwich assay; Silica nanoparticles
- Issue Date
- Biosensors & bioelectronics
- VOL 25, 130-135
- We report Hepatitis B Virus (HBV) DNA detection using a silica nanoparticle-enhanced dynamic microcantilever
biosensor. A 243-mer nucleotide of HBV DNA precore/core region was used as the target DNA.
For this assay, the capture probe on the microcantilever surface and the detection probe conjugated with
silica nanoparticleswere designed specifically for the target DNA. For efficient detection of the HBV target
DNA using silica nanoparticle-enhanced DNA assay, the size of silica nanoparticles and the dimension of
microcantilever were optimized by directly binding the silica nanoparticles through DNA hybridization.
In addition, the correlation between the applied nanoparticle concentrations and the resonant frequency
shifts of the microcantileverwas discussed clearly to validate the quantitative relationship between mass
loading and resonant frequency shift.
HBV target DNAs of 23.1 fMto 2.31 nMwhichwere obtained fromthe PCR productwere detected using
a silica nanoparticle-enhanced microcantilever. The HBV target DNA of 243-mer was detected up to the
picomolar (pM) level without nanoparticle enhancement and up to the femtomolar (fM) level using a
nanoparticle-based signal amplification process. In the above two cases, the resonant frequency shifts
were found to be linearly correlated with the concentrations of HBV target DNAs. We believe that this
linearity originated mainly from an increase in mass that resulted from binding between the probe DNA
and HBV PCR product, and between HBV PCR product and silica nanoparticles for the signal enhancement,
even though there is another potential factor such as the spring constant change that may have influenced
on the resonant frequency of the microcantilever.
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