Detection of Hepatitis B Virus (HBV) DNA at femtomolar concentrations using a silica nanoparticle-enhanced microcantilever sensor

Title
Detection of Hepatitis B Virus (HBV) DNA at femtomolar concentrations using a silica nanoparticle-enhanced microcantilever sensor
Authors
차병학이상명박재찬황교선김상경이윤식주병권김태송
Keywords
Dynamic microcantilever; HBV DNA; Sensitivity enhancement; Sandwich assay; Silica nanoparticles
Issue Date
2009-08
Publisher
Biosensors & bioelectronics
Citation
VOL 25, 130-135
Abstract
We report Hepatitis B Virus (HBV) DNA detection using a silica nanoparticle-enhanced dynamic microcantilever biosensor. A 243-mer nucleotide of HBV DNA precore/core region was used as the target DNA. For this assay, the capture probe on the microcantilever surface and the detection probe conjugated with silica nanoparticleswere designed specifically for the target DNA. For efficient detection of the HBV target DNA using silica nanoparticle-enhanced DNA assay, the size of silica nanoparticles and the dimension of microcantilever were optimized by directly binding the silica nanoparticles through DNA hybridization. In addition, the correlation between the applied nanoparticle concentrations and the resonant frequency shifts of the microcantileverwas discussed clearly to validate the quantitative relationship between mass loading and resonant frequency shift. HBV target DNAs of 23.1 fMto 2.31 nMwhichwere obtained fromthe PCR productwere detected using a silica nanoparticle-enhanced microcantilever. The HBV target DNA of 243-mer was detected up to the picomolar (pM) level without nanoparticle enhancement and up to the femtomolar (fM) level using a nanoparticle-based signal amplification process. In the above two cases, the resonant frequency shifts were found to be linearly correlated with the concentrations of HBV target DNAs. We believe that this linearity originated mainly from an increase in mass that resulted from binding between the probe DNA and HBV PCR product, and between HBV PCR product and silica nanoparticles for the signal enhancement, even though there is another potential factor such as the spring constant change that may have influenced on the resonant frequency of the microcantilever.
URI
http://pubs.kist.re.kr/handle/201004/35697
ISSN
0956-5663
Appears in Collections:
KIST Publication > Article
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