OPTIMIZATION OF BUTANOL PRODUCTION OF CLOSTRIDIUM PASTEURIANUM USING RESPONSE SURFACE METHODOLOGY
- OPTIMIZATION OF BUTANOL PRODUCTION OF CLOSTRIDIUM PASTEURIANUM USING RESPONSE SURFACE METHODOLOGY
- 문철우; 상병인; 엄영순
- glycerol; butanol; Response Surface Methodology(RSM); Clostridium pasteurianum DSM525; 1,3-propanediol
- Issue Date
- 8th World Congress of Chemical Engineering
- In this study, the optimization of fermentation conditions for converting glycerol to butanol was investigated with Clostridium pasteurianum DSM525, which is known to convert glycerol to butanol and 1,3-propanediol (1,3-PD). Based on our ‘one-factor-at-a-time’ studies, pH, glycerol concentration, and the addition of co-substrate such as butyrate significantly influenced cell growth and butanol production by C. pasteurianum DSM525 in batch cultures. When 2-(N-Morpholino) ethanesulfonic acid (MES) was added to reduce pH drop during batch fermentations, butanol production was higher than 1,3-PD production. Without MES addition, however, butanol concentration was lower than 1,3-PD. The reason for those far different patterns of butanol production with and without MES would be pH control effect in the presence of MES. The addition of co-substrate, such as butyrate, was found to enhance the production of butanol by more than 50%. The concentration of carbon source, glycerol, also significantly affected cell growth and butanol production. Accoring to our results, pH, the concentration of glycerol and butyrate are lilkly to be tree important variables to impact butanol production of C. pasteurianum DSM525. Respose Surface Methodology was employed to analyze responses when different conditions of those three variables were applied. Further screening experiments will be also done to identify other important variables that significantly influence butanol production of C. pasteurianum DSM525.
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