The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism

Title
The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism
Authors
박인수한민이종원김수현정영미김익환김상헌
Issue Date
2009-09
Publisher
TERMIS-WC2009
Abstract
We sought to determine the adhesive properties of human adipose-derived stem cells (hASCs) for extracellular matrix proteins. Cell adhesion on fibronectin (Fn) was inhibited by the heparin-binding peptide (HBP) in the presence of Mab 2253, but not by either Mab 2253 or HBP alone. These results indicate that both the b1 subunit and the heparin sulfate proteoglycan participated in the cell adhesion to Fn. Microscopic views showed extensive spreading of hASCs cultured on Fn, whereas the cells maintained a round shape when cultured on a heparin-binding domain (HBD) substrate. hASCs differentiated into adipocytes, which stained positive for lipid vacuoles by Oil Red-O analysis, more readily on HBD substrate than on FN substrate. Taken together, these results suggest that hASCs have an adhesion mechanism for the HBD of Fn and hASC morphology is controlled by the adhesion mechanism and strongly correlated with adipogenic differentiation.
URI
http://pubs.kist.re.kr/handle/201004/35834
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KIST Publication > Conference Paper
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