The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism
- The correlation between human adipose-derived stem cells differentiation and cell adhesion mechanism
- 박인수; 한민; 이종원; 김수현; 정영미; 김익환; 김상헌
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- We sought to determine the adhesive properties of human adipose-derived stem cells (hASCs) for extracellular matrix proteins. Cell adhesion on fibronectin (Fn) was inhibited by the heparin-binding peptide (HBP) in the presence of Mab 2253, but not by either Mab 2253 or HBP
alone. These results indicate that both the b1 subunit and the heparin sulfate proteoglycan
participated in the cell adhesion to Fn. Microscopic views showed extensive spreading of
hASCs cultured on Fn, whereas the cells maintained a round shape when cultured on a heparin-binding domain (HBD) substrate. hASCs differentiated into adipocytes, which stained
positive for lipid vacuoles by Oil Red-O analysis, more readily on HBD substrate than on FN
substrate. Taken together, these results suggest that hASCs have an adhesion mechanism for
the HBD of Fn and hASC morphology is controlled by the adhesion mechanism and strongly
correlated with adipogenic differentiation.
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