Inhibitory Effects of 23 Korean Local Plant Extracts on Aldo-keto reductase family 1 B10 (AKR 1 B10)

Title
Inhibitory Effects of 23 Korean Local Plant Extracts on Aldo-keto reductase family 1 B10 (AKR 1 B10)
Authors
이주영송대근정상훈김종환안수용노주원판철호
Keywords
Aldo-keto reductase family 1 B10; aldose reductase-like 1; anti-cancer; Ligularia fischeri; Rhus trichocarpa
Issue Date
2009-10
Publisher
생약학회지
Citation
VOL 40, NO 3, 238-243
Abstract
We examined ethanol extracts prepared from 23 Korean local plants obtained in Pyeongchang, Gangwon-do for their inhibitory effects on recombinant human AKR 1 B10 (rhAKR1B10) in vitro. To do this, rhAKR1B10 was first expressed in E. coli as a biological active form and purified by using Ni-affinity chromatography followed by gel permeation chromatography. Then, rhAKR1B10 was used for screening out 23 Korean local plant extracts having an inhibitory activity against itself. Among them, six extracts showed more than 50% inhibition of rhAKR1B10 activity at the concentration of 10μg/ml. Especially, the extracts of Ligularia fischeri var. spiciformis Nakai and Rhus trichocarpa Miq. were the most potent because their IC50 values were 2.94 and 2.00 μg/ml, respectively.
URI
http://pubs.kist.re.kr/handle/201004/35939
ISSN
0253-3073
Appears in Collections:
KIST Publication > Article
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