Detoxification of model phenolic compounds in lignocellulosic hydrolysates with peroxidase for butanol production from Clostridium beijerinckii
- Detoxification of model phenolic compounds in lignocellulosic hydrolysates with peroxidase for butanol production from Clostridium beijerinckii
- 조대행; 이윤지; 엄영순; 상병인; 김용환
- Lignocellulosic hydrolysates; Phenolic compound; Peroxidase; Butanol; Clostridium beijerinckii; Inhibitor
- Issue Date
- Applied microbiology and biotechnology
- VOL 83, NO 6, 1035-1043
- In the present study, we investigated the peroxidasecatalyzed
detoxification of model phenolic compounds and
evaluated the inhibitory effects of the detoxified solution on
butanol production by Clostridium beijerinckii National
Collection of Industrial and Marine Bacteria Ltd. 8052. The
six phenolic compounds, p-coumaric acid, ferulic acid, 4-
hydroxybenzoic acid, vanillic acid, syringaldehyde, and
vanillin, were selected as model fermentation inhibitors
generated during pretreatment and hydrolysis of lignocellulose.
The enzyme reaction was optimized as a function of the
reaction conditions of pH, peroxidase concentration, and
hydrogen peroxide to substrate ratio. Most of the tested
phenolics have a broad optimum pH range of 6.0 to 9.
Removal efficiency increased with the molar ratio of H2O2 to
each compound up to 0.5–1.25. In the case of p-coumaric
acid, ferulic acid, vanillic acid, and vanillin, the removal
efficiency was almost 100% with only 0.01 μM of enzyme.
The tested phenolic compounds (1 g/L) inhibited cell growth
by 64–74%, while completely inhibiting the production of
butanol. Although syringaldehyde and vanillin were less toxic
on cell growth, the level of inhibition on the butanol production
was quite different. The detoxified solution remarkably
improved cell growth and surprisingly increased butanol
production to the level of the control. Hence, our present
study, using peroxidase for the removal of model phenolic
compounds, could be applied towards the detoxification of
lignocellulosic hydrolysates for butanol fermentation.
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