Simultaneous quantitative determination of urinary cholesterol, desmosterol and lanosterol in pravastatin treated rats by gas chromatography/mass spectrometry

Title
Simultaneous quantitative determination of urinary cholesterol, desmosterol and lanosterol in pravastatin treated rats by gas chromatography/mass spectrometry
Authors
사릴꾸마르정봉철이영주이홍재정병화
Keywords
cholesterol; lanosterol; desmosterol; GC/MS; pravastatin
Issue Date
2009-10
Publisher
분석과학; Analytical Science & Technology
Citation
VOL 22, NO 5, 407-414
Abstract
A simultaneous determination method for cholesterol, lanosterol and desmosterol was developed using gas chromatography/mass spectrometry. Urine was enzymatically hydrolyzed with β-glucuronidase/arylsulfatase. Samples were prepared using extractions with a mixture of ethyl acetate-hexane (2:3, v/v), followed by derivatization with a mixture of MSTFA/ TMSI/ TMCS (100:2:5 v/v/v). All analyses were performed using GC/ MS in selective ion monitoring mode. Good linearities (r2=0.998~0.999) in calibration curve and a satisfactory recovery (80.0%~113%) were achieved. Accuracy and precision values within ±15% in the concentration range of 5 to 200 ng/mL were also observed for all compounds. The developed method was applied to pravastatintreated (70 and 250 mg/kg/day for 7 days, oral) hyperlipidemia rats. Those sterols were significantly lower in drug-treated rats compared to the controls, which justifies the drug efficacy. Therefore, these results indicate that the developed method was successfully applied to examine statin drug efficacy with urine sample.
URI
http://pubs.kist.re.kr/handle/201004/36065
ISSN
1225-0163
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KIST Publication > Article
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