Hyaluronic acid complexed to biodegradable poly L-arginine for targeted delivery of siRNAs

Title
Hyaluronic acid complexed to biodegradable poly L-arginine for targeted delivery of siRNAs
Authors
김은중심가용김광명권익찬오유경심창구
Keywords
biodegradable cationic polymer; hyaluronic acid; poly L-arginine; siRNA delivery
Issue Date
2009-09
Publisher
The journal of gene medicine
Citation
VOL 11, NO 9, 791-803
Abstract
Gel retardation assays showed that HP101 and HP110 could form complexes with siRNAs. The diameters of these complexes were less than 200 nm. Cellular delivery efficiency of siRNAs by HPs depended on cell surface CD44 density. The HP-mediated delivery of siRNAs was highest in WM266.4 cells followed by B16F10 cells and COS-7 cells, in parallel with CD44 surface densities of these cell lines. TC50 values (i.e. the HP concentrations at which 50% of cells were viable after treatment) were used as indicators of cytotoxicity. HP101 showed TC50 values that were 2-fold and 23-fold higher than those of HP110 and PLR, respectively. After delivery into cells, siRNA exerted target-specific RNA interference effects on mRNA and protein levels. Three days after treatment of red fluorescent protein (RFP)-expressing B16F10 cells with RFP-specific siRNA complexed to HP101, cellular fluorescence signals were reduced. Intratumoral administration of RFP-specific siRNA via HP101 delivery significantly reduced the expression of RFP in tumor tissues.
URI
http://pubs.kist.re.kr/handle/201004/36085
ISSN
1099-498X
Appears in Collections:
KIST Publication > Article
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