Design and characterization of a maltose binding protein-linked growth factor for matrix engineering

Title
Design and characterization of a maltose binding protein-linked growth factor for matrix engineering
Authors
한민박인수김수현김병수김상헌
Keywords
Artificial matrix; Cell adhesion; Growth factor; Maltose-binding protein
Issue Date
2009-10
Publisher
Biotechnology letters
Citation
VOL 31, 1677-1684
Abstract
The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 1019 ng/cm2 at 100 lg protein/ml. The phosphorylation of VEGF receptors in the MBP-VEGF stimulated HEK293/KDR cells as depicted from western blot analysis. Cell adhesion to a MBP-VEGF immobilized surface was mediated by the VEGF-VEGFR interaction. These results suggest that MBP-VEGFs are active and a MBP immobilization system can then anchor various bioactive proteins to hydrophobic surfaces.
URI
http://pubs.kist.re.kr/handle/201004/36107
ISSN
0141-5492
Appears in Collections:
KIST Publication > Article
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