A Cell-based Method to Monitor the Interaction between Hepatitis B Virus Capsid and Surface Proteins

Title
A Cell-based Method to Monitor the Interaction between Hepatitis B Virus Capsid and Surface Proteins
Authors
김윤경오수진진봉석박찬후전혜성부두완유연규
Keywords
HBV; Capsid; Interaction; PreS
Issue Date
2009-03
Publisher
Bulletin of the Korean Chemical Society
Citation
VOL 30, NO 3, 577-581
Abstract
Interactions between the surface and capsid proteins of the hepatitis B virus (HBV) are critical for the assembly of virus particles. In this study, we developed a cell-based method to visualize the interactions between the capsid and surface proteins of HBV. Capsid-GFP, a capsid protein fused to a green fluorescence protein (GFP), forms nucleocapsid-like structures in the cytoplasm of mammalian cells. It relocates to the plasma membranes in cells expressing PH-PreS, a fusion protein consisting of the PreS region of the HBV surface protein and the PH domain of PLC-γ. Membrane localization of the capsid-GFP in these cells is prevented by an inhibitory peptide that blocks the interaction between the capsid and surface proteins. This dynamic localization of capsid-GFP is applicable for screening compounds that may potentially inhibit or prevent the assembly process of HBV particles.
URI
http://pubs.kist.re.kr/handle/201004/36117
ISSN
0253-2964
Appears in Collections:
KIST Publication > Article
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