A Cell-based Method to Monitor the Interaction between Hepatitis B Virus Capsid and Surface Proteins
- A Cell-based Method to Monitor the Interaction between Hepatitis B Virus Capsid and Surface Proteins
- 김윤경; 오수진; 진봉석; 박찬후; 전혜성; 부두완; 유연규
- HBV; Capsid; Interaction; PreS
- Issue Date
- Bulletin of the Korean Chemical Society
- VOL 30, NO 3, 577-581
- Interactions between the surface and capsid proteins of the hepatitis B virus (HBV) are critical for the assembly of
virus particles. In this study, we developed a cell-based method to visualize the interactions between the capsid and
surface proteins of HBV. Capsid-GFP, a capsid protein fused to a green fluorescence protein (GFP), forms
nucleocapsid-like structures in the cytoplasm of mammalian cells. It relocates to the plasma membranes in cells
expressing PH-PreS, a fusion protein consisting of the PreS region of the HBV surface protein and the PH domain
of PLC-γ. Membrane localization of the capsid-GFP in these cells is prevented by an inhibitory peptide that blocks
the interaction between the capsid and surface proteins. This dynamic localization of capsid-GFP is applicable for
screening compounds that may potentially inhibit or prevent the assembly process of HBV particles.
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