Chemopreventive dual-effect of Adenocaulon himalaicum through induction of apoptosis and detoxification enzyme
- Chemopreventive dual-effect of Adenocaulon himalaicum through induction of apoptosis and detoxification enzyme
- 이샛별; 강경수; 윤지호; 이희주; 김철영; 노주원
- Adenocaulon himalaicum; apoptosis; detoxification enzyme; NRF2
- Issue Date
- 2009 한국분자세포생물학회
- Cancer chemoprevention is classified into two categories either by blocking of the initiation or suppressing of the promotion during carcinogenesis. Phase II detoxification enzymes are known to play essential roles in blocking step. Apoptosis is also an important target for inhibiting tumor promotion. In this study, we have investigated the cancer preventive activity of the ethanolic extract of A. hialaicum (AHE) and its butanol fraction (AHB). AHE and AHB induce QR activity having a relatively high chemoprevention index (CI=12.4), and increased NQO1, HO-1 mRNA levels through Nrf2-ARE pathway in HCT116 cells. Moreover, AHB increased CYP1A1 expression and activity, and increased Nrf2 mRNA levels maximum about 8-fold. We also found that AHE and AHB induced apoptosis, as evidenced by the externalization of phosphatidylserine, chromatin condensation, PARP cleavage, and induction of p53. These data suggest that the chemopreventive effects of AHE and AHB could be resulted from both induction of detoxification enzymes and apoptosis. Thus, A. himalaicum could be developed as a potential cancer chemopreventive agent for prevention or treatment of human cancers.
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