Bifunctional induction of youngiaside A, B and C for Cancer Chemoprevention through the both Nrf2/ARE and AhR/XRE Pathway in Caco-2 Human Colorectal Cancer Cells
- Bifunctional induction of youngiaside A, B and C for Cancer Chemoprevention through the both Nrf2/ARE and AhR/XRE Pathway in Caco-2 Human Colorectal Cancer Cells
- 윤지호; 이샛별; 이희주; 김철영; 김미애; 손영창; 노주원
- Youngiasides; AhR; Nrf2; Quinone Reductase
- Issue Date
- 2009 분자세포생물학회
- Many phytochemicals are the induction of phase I and/or phase II detoxification enzymes for cancer chemoprevention. The one of the plays critical roles are prevention against chemical carcinogens or toxic xenobiotics through detoxification enzymes. In this study, we investigated that cancer chemopreventive activity of youngiaside A, B and C (YA, YB and YC) from Crepidiastrum denticulatum in Hepa1c1c7 cells. The YA, YB and YC significantly induced QR activity and relatively showed high chemoprevention indices (CI). Also SEAP activity was induced in Hepa-QR-SEAP cells (a stable cell; contains whole promoter region of QR). In addition, ARE and XRE, a part of promoter region for QR gene expression, was significantly activated in Caco-2 cells. Moreover, these ARE and XRE activation was resulted from induction of translocates into nucleus of Nrf2 and AhR as well as significantly increased mRNA and protein expression of both NQO1 and CYP1A1 in Caco-2 cells. These result showed that the YA, YB and YC were induced QR activity through Nrf2/ARE and AhR/XRE pathway. Thus, bifunctional inducer as YA, YB and YC could be potential agents for cancer prevention.
Moreover, YA, YB and YC increased NQO1 and CYP1A1 mRNA level and protein expression through both Nrf2/ARE and AhR/XRE pathway in Caco-2 cells. ARE and XRE were cis-acting elements in promoter region of QR. Thus, bifunctional inducer as YA, YB and YC could be potential agents for cancer prevention.
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