Inhibitory Effects of Korean Local Plant Extracts on Aldo-keto reductase 1B10 (AKR1B10)

Title
Inhibitory Effects of Korean Local Plant Extracts on Aldo-keto reductase 1B10 (AKR1B10)
Authors
이주영송대근정상훈노주원손진기판철호
Keywords
aldo-keto reductase; plant extract; enzyme inhibition; protein expression; protein purification; AKR1B10; cancer prevention
Issue Date
2009-10
Publisher
한국응용생명화학회
Abstract
We examined ethanol extracts prepared from 113 Korean local plants obtained in Pyeongchang, Gangwon-do for their inhibitory effects on recombinant human AKR1B10 (rhAKR1B10) in vitro. To do this, rhAKR1B10 was first expressed in E.coli as a biological active from and purified by using Ni-affinity chromatography followed by gel permeation chromatography. Then, rhAKR1B10 was used for screening out 113 Korean local plant extracts having inhibition activity against itself. Among them, 21 extracts showed more than 50% inhibition of rhAKR1B10 activity at the concentration of 10 μg/mL. Especially, the extracts of Ligularia fischeri var. spiciformis Nakai and Rhus trichocarpa Miq. Were the most potent because their IC50 values were 2.94 and 2.00 μg/mL, respectively.
URI
http://pubs.kist.re.kr/handle/201004/36379
Appears in Collections:
KIST Publication > Conference Paper
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